When protease subgroup is unknown the group number of proposed cleavage substrate (hydrogenase) is written in brackets. It is based on the MK0683 ic50 protease’s placement within the phylogenetic tree, the number of hydrogenases within each strain and the possibility for co-transcription

with a hydrogenase. X: The point in the phylogenetic tree when horizontal gene transfer might have occurred. Y/Z: learn more Suggested positions of root. Archaean strains: red text. Bacterial strains: black text. For abbreviations used see Additional file 2. The tree were constructed using the MrBayes software which was executed for 1 500 000 generations with a sample frequency of 100 using the WAG model. A burn-in of 3750 (25%) trees was used. For graphic outputs the resulting trees were visualised by using Treeview. (PDF 267 KB) Additional file 2: Table organisms. This excel-file contains a table of all hydrogenase specific proteases used in the extended phylogenetic tree (Additional file 1) including strain, organism, locus_tag, abbreviation,

accession number, and proposed phylogenetic group. This file also contains the number of hydrogenases in each strain including accession number. Proposed cleavage substrate (hydrogenase large subunit) for each protease is marked with grey background/bold 4SC-202 in vitro text and is based on each protease position in phylogenetic tree, the number of hydrogenases within each strain and location within genome (i.e. possibility for co-transcription with hydrogenase gene). B; unknown phylogenetic group. (XLS 34 KB) Additional file 3: Alignment NpunF0373homolgoues. This word document file shows an alignment of NpunF0373 and homologues found in other organisms, all cyanobacterial strains, including locus_tag and accession number. Inositol monophosphatase 1 (DOC 42 KB) Additional file 4: Supplementary figure NpunF0373homologoues. This word document file show the presence/absence of homologous to the gene Npun_F0373 of Nostoc punctiforme in selected cyanobacterial strains together with their, when present, locus_tag and GenBank accession number. hupL,

hupW, hoxH, hoxW and different metabolic functions; the ability to produce heterocyst and filaments and the capacity for nitrogen-fixation, are also indicated. (+); present, (-); absent, (?); presence/absence unknown. (DOC 44 KB) References 1. Tomitani A, Knoll AH, Cavanaugh CM, Ohno T: The evolutionary diversification of cyanobacteria: molecular-phylogenetic and paleontological perspectives. Proc Natl Acad Sci USA 2006,103(14):5442–5447.CrossRefPubMed 2. Cavalier-Smith T: Cell evolution and Earth history: stasis and revolution. Philos Trans R Soc Lond B Biol Sci 2006,361(1470):969–1006.CrossRefPubMed 3. Tamagnini P, Leitao E, Oliveira P, Ferreira D, Pinto F, Harris DJ, Heidorn T, Lindblad P: Cyanobacterial hydrogenases: diversity, regulation and applications. FEMS Microbiol Rev 2007,31(6):692–720.CrossRefPubMed 4. Dunn JH, Wolk CP: Composition of the cellular envelopes of Anabaena cylindrica.