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“It is not clear whether antipseudomonal agents Chk inhibitor can kill cell-associated bacteria within a short time. Madin-Darby canine kidney (MDCK) and A549 cells were infected with Pseudomonas aeruginosa ATCC 27853 and PAO1 and the bactericidal activity of ceftazidime, imipenem, meropenem, gentamicin, and ciprofloxacin against the organisms was investigated. In both MDCK and A549 cells, beta-lactams could not kill epithelial cell-associated bacteria within 2 h. Gentamicin at concentrations a parts per

thousand currency sign32 mu g/ml killed more than 99% of epithelial cell-associated bacteria. Ciprofloxacin at 0.5 mu g/ml killed more than 99.9% of MDCK cell-associated bacteria. Ciprofloxacin has the strongest and most rapid bactericidal activity against epithelial cell-associated bacteria, which may be explained by the combination of potent in-vitro bactericidal activity and high penetration ability into epithelial cells.”
“Purpose: To investigate the antimicrobial activity of crude and fractionated extracts of Biemna tubulosa and Stylissa spp. thriving in Trou aux Biches lagoon, North East Mauritius.

Methods: Crude extracts (methanol: dichloromethane 1: 1) and fractions of Biemna tubulosa and Stylissa spp (hexane, ethyl acetate and butanol)

were tested against some bacterial organisms, namely, Escherichia coli, Staphylococcus aureus and Enterococcus faecalis, using broth microdilution assay. The extracts were screened for the presence of compounds that could be responsible for bioactivity.

Results: Preliminary chemical screening revealed the presence of tannins, saponins, terpenes, alkaloids and phenols. Cell Cycle inhibitor The hexane fraction of Stylissa spp. and the ethyl actetate and butanol extracts of Biemna tubulosa were more active than the control antibiotic. Maximum antimicrobial activity was noted for the butanol fraction of Biemna tubulosa with minimum inhibitory concentration (MIC)

of 0.091 mg/ml against E. coli. The hexane soluble fraction of Stylissa spp. was more active with MIC of 0.125 mg/ml against S. aureus and E. coli and 0.250 mg/ml against E. faecalis.

Conclusion: The broad selleck screening library spectrum antibacterial activity of these sponges seemed to be due to the presence of sesterterpenes and triterpenes, alkaloids and tannins detected in the extract fractions. This is probably the first report on the antimicrobial activity of Biemna tubulosa and Stylissa spp. from Mauritius waters.”
“Rupture of the upper ankle joint ligaments is a common injury. Therapy in patients with Ehlers Danlos Syndrome (EDS) is a challenge due to the autosomal-dominant collagenopathy. Therapeutic recommendations and long term results in treatment of patients with EDS are rare. Therefore uncertainty exists. We present a case of clinical and radiologic long term results after ligament rupture of the upper ankle joint in a patient with EDS.