Confidence intervals of S i,res

indicate that the fraction of virus that can survive thermal treatment differs depending on the titration method used and the temperature. With EMA-IGEPAL CA-630 – RT-qPCR and RT-qPCR assay C, the S 2,res value is approximately −1.6 log10, which means that 1 virus out of 40 is quantifiable after 20 min of treatment regardless of the temperature. With EMA-IGEPAL CA-630 – RT-qPCR and RT-qPCR assay A or B, between 1 virus out 200 and 1 virus out of 6000 is still quantifiable after treatment at 68°C and 80°C (with S 2,res ranged between −2.3 log10 and −3.8 log10). For RT-qPCR, S 1,res are much higher than S 2,res, but the difference between RT-qPCR assays A and B and RT-qPCR assay C was also observed for RT-qPCR. For the infectious titration method,

S 3,res is around −3.5 log10, LY333531 order close to the values obtained with EMA-IGEPAL CA-630 – RT-qPCR associated with RT-qPCR assays A and B. For RV strains, the values of S2,0 were lower than zero, which means that the EMA / PMA treatment affected virus quantification with regard to the RT-qPCR method. Indeed, the reduction of the concentration of infectious virus due to the monoazide pre-treatment was about of −0.5 log10 by using RT-qPCR assay A and is ranged from −1.2 log10 to −2.5 log10 by using RT-qPCR assays B and C. These reduction levels were RXDX-101 mw the same for both RV strains. At 37°C, the level of RV strains remained constant regardless of the method used. At 68°C, 72°C, and 80°C, the genomic titer of the RV strains was found to be constant by using the RT-qPCR method regardless of the RT-qPCR assay tested. The S i,res confidence intervals indicate that the fraction of virus that can survive thermal treatment differs depending on the titration method used. For the Wa RV strain, with EMA-RT-qPCR and RT-qPCR assay A, the S 2,res value was approximately −1.3 log10 which means 1 virus out of 20 was quantifiable after 20 min of treatment regardless of the temperature. With EMA-RT-qPCR and RT-qPCR

assays B or C, between 1 virus out of 104 and 1 virus out of 105, was still quantifiable after treatment at 68°C, 72°C or 80°C (i.e. S 2,res ranged between −4 log10 and Farnesyltransferase −5 log10). The S3,res values obtained with the infectious titration method were similar to the S 2,res values of RT-qPCR assays B and C. For the SA11 RV strain, with PMA-RT-qPCR and RT-qPCR assay A, S 2,res value is approximately −1.2 log10. With RT-qPCR assays B and C, S 2,res ranged from −2.4 log10 and −3.9 log10. The value of S 2,res with these RT-qPCR assays decreased significantly when the temperature of treatment increases. S3,res values cannot be estimated as inactivation after 1 minute of treatment for 68°C, 72°C or 80°C was higher than the LOQ.