We next examined whether the chronic opiate-induced morphological

We next examined whether the chronic opiate-induced morphological change was correlated with changes in DA neuronal excitability. check details We found that chronic morphine-treated mice, compared with sham-treated mice, exhibited an increase in the spontaneous firing rate of VTA DA neurons in brain slices (Figure 1C). This effect was not dependent on residual morphine in the slice, since blockade of opioid receptors with naloxone did not affect cell excitability

(Figure 1C). Moreover, the inclusion of a low dose of morphine (5 μM) in the bath solution to prevent “withdrawal” in the slice did not alter DA neuron firing rate (Figure 1C). Given the observations that chronic morphine decreases the size of VTA selleck chemicals DA neurons, but concomitantly increases their excitability, it was important to determine whether net DA output from VTA is altered. We examined levels of extracellular DA in nucleus accumbens (NAc) in vivo, widely considered a key determinant of reward (Hyman et al., 2006). In opposition

to the increased firing rate, we found that chronic morphine dramatically decreased electrically evoked DA output in NAc of rats as measured by fast-scan cyclic voltammetry (Figure 1D). This reduction in DA output from VTA DA neurons supports the notion that the reduced soma size of the neurons, induced by chronic morphine, correlates with functional output, consistent with the reward MycoClean Mycoplasma Removal Kit tolerance induced by chronic morphine under these conditions (Russo et al., 2007). Next, we examined a possible relationship between the increase in VTA DA neuron firing rate and soma size decrease, with the hypothesis that the increased firing rate per se induces changes in soma size. We virally overexpressed

a dominant-negative K+ channel subunit (dnK, KCNAB2-S188A, R189L) locally within VTA; we showed previously that this mutant channel increases the firing rate of VTA DA neurons (Krishnan et al., 2007). Overexpression of dnK was sufficient to decrease the surface area of VTA DA neurons (Figure 2A). To obtain the converse type of information, we virally overexpressed wild-type Kir2.1 in VTA, which we showed decreases DA neuron firing rate (Krishnan et al., 2007). While overexpression of Kir2.1 alone did not affect VTA DA soma size (data not shown), it completely blocked the ability of chronic morphine both to decrease soma size (Figure 2B) and to increase DA neuron firing rate (Figure 2C). These findings support our hypothesis that the morphine-induced increase in VTA DA neuron excitability is both necessary and sufficient for mediating the decrease in soma size. Given the increase in VTA DA neuronal firing rate observed in response to chronic morphine, we examined possible underlying mechanisms. One possibility is that morphine, by downregulating AKT activity in these neurons (Russo et al.

Leave a Reply

Your email address will not be published. Required fields are marked *

*

You may use these HTML tags and attributes: <a href="" title=""> <abbr title=""> <acronym title=""> <b> <blockquote cite=""> <cite> <code> <del datetime=""> <em> <i> <q cite=""> <strike> <strong>