We also tried to identify the origin of metanephric mesenchyme by lineage trace experiments utilizing T-GFPCreER mice. Next, we examined the combinations of factors which are required for

metanephric nephron progenitor specification from embryonic nascent mesoderm. Finally, by applying this protocol, we tried to establish the way to induce metanephric nephron progenitors and reconstitute three-dimensional kidney structures from PSCs. Results: We identified that the MM is originated from posteriorly located T+ precursors at embryonic day (E) 8.5, and that developmentally distinct from Osr1+ UB progenitors. T+ cells sorted from mouse embryos differentiate into the metanephric mesenchyme in vitro by posteriorization with a high concentration of PD-332991 Wnt agonist, followed by its graded attenuation and stage-specific growth factor addition. When mouse and human pluripotent stem cells were treated similarly, metanephric nephron progenitors were obtained that could reconstitute the three-dimensional structures of the kidney, including glomeruli with podocytes and renal tubules with proximal and distal regions and clear lumina. Furthermore, the glomeruli were

efficiently vascularized upon transplantation. ALK activation Conclusion: We have succeeded in inducing the metanephric nephron progenitors from both mouse embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells, in vitro. The resultant progenitors readily formed the three-dimensional structures of the kidney, comprising renal tubules and notably glomeruli with podocytes, which has not previously been achieved. Thus, by re-evaluating the developmental origins of metanephric progenitors, we have provided key insights into kidney specification in vivo and taken important steps toward kidney organogenesis

in vitro. LIU CHUN-BEI1,2, KANAMARU YUTAKA1, WATANABE TOMONARI1, TADA NOBUHIRO3, Amrubicin SUZUKI YUSUKE1, TOMINO YASUHIKO1 1Division of Nephrology, Department of Internal Medicine, Juntendo University Faculty of Medicine, Tokyo; 2Research Institute of Nephrology, Jinling Hospital, Medical School of Nanjing University, Nanjing, China; 3Juntendo University Research Institutefor Diseases of Old Ages Introduction: Macrophages are crucial for the formation and progression of atherosclerosis. OxLDL, which could be accumulated by macrophage to form foam cell, has been described to promote atherosclerosis by the activation of mitogen-activated protein kinase(MAPK) induced phosphorylation evens. Whether FcαRI target therapy by monoantibody of FcαRI, which effectively inhibit MAPK pathway, could influence the progression of atherosclerosis is unclear. Methods: We constructed ApoE-deficient mice expressing human FcαRI/g and did a 2 times/month’s spleen macrophage transfer from these mice to APOE−/− mouse. Atherosclerosis was promoted by high fat diet for 3 months and FcαRI target therapy was given simultaniously for same duration.