Transcellular uptake of elaidic acid (t18:1), a major trans-fatty acid present in processed foods, in Caco-2 monolayers was significantly reduced by 28.3 and 16.7% 60 min after WBE and BBE treatment, respectively. Results of flow cytometry (FACS) analysis showed significant reductions in boron-dipyrromethene
(BODIPY) fluorescence-labeled fatty acid uptake by 35.4 and 40.2% with WBE and S3I-201 cell line BBE treatment, respectively. BBE treatment significantly reduced the expression of fatty acid transport protein-4 and CD36 in Caco-2 cells, as determined by quantitative real time-polymerase chain reaction (qRT-PCR). Similar trends were found in WBE treatment, although to a lesser degree. These observations suggest that soybean extract may reduce fatty acid uptake and cellular fat accumulation by altering fatty acid transporter expression.”
“Background: Reducing circulating levels of fibroblast growth factor-23 (FGF-23) in patients on renal replacement GANT61 nmr therapy (RRT) could appear as an appealing approach for cardiovascular events prevention.
The purpose of our study was to compare the clearance of FGF-23 between high flux hemodialysis (HD) and online hemodiafiltration (OL-HDF) procedures.
Methods: Fifty-three patients were included in the HD group while 32 patients were included in the OL-HDF group. Serum pre- and post-dialysis FGF-23 levels were measured using a 2nd generation Human FGF-23 (C-Term) ELISA kit.
Results: Mean FGF-23 reduction rates were significantly higher in OL-HDF (55.7 +/- 25.2%) versus HD (36.2 +/- 28.6%) groups (P=.0001), as were calculated FGF-23 clearances (P=.015) and Kt/V-FGF-23 (P=.001). Overall, post-treatment FGF-23 levels were significantly lower than pre-treatment values in both groups (P<.01).
Conclusions: OL-HDF achieves higher FGF-23 removal over high flux HD.”
“Recent studies have suggested that the pineal Nutlin-3 manufacturer hormone melatonin may protect against breast cancer, and the mechanisms
underlying its actions are becoming clearer. Melatonin works through receptors and distinct second messenger pathways to reduce cellular proliferation and to induce cellular differentiation. In addition, independently of receptors melatonin can modulate oestrogen-dependent pathways and reduce free-radical formation, thus preventing mutation and cellular toxicity. The fact that melatonin works through a myriad of signalling cascades that are protective to cells makes this hormone a good candidate for use in the clinic for the prevention and/or treatment of cancer. This review summarises cellular mechanisms governing the action of melatonin and then considers the potential use of melatonin in breast cancer prevention and treatment, with an emphasis on improving clinical outcomes.”
“A unique antioxidant peptidoglycan of 6.5 kDa was purified from silk worm (Bombyx mori) pupae.