(See Supplementation Protocol Section). Subjects were directed to continue the same general lifestyle patterns of exercise and nutritional intake during each seven-day period prior to the two exercise testing sessions. To verify the consistency of training and diet, the subjects were directed to complete a 7-day exercise log and a 3-day dietary recall (two week days and one weekend day) for each week prior to testing. The exercise log provided information regarding the volume (sets and reps) of resistance training relative to upper body, lower body, or total body structural movements. The dietary intake information was analyzed using ESHA Food Processor SQL dietary analysis software (ESHA Research, Salem,

OR). All research participants completed at least two familiarization trials prior GSK2245840 cell line to participating in the two testing sessions. The familiarization sessions followed the same general protocol but without full measurements of the actual selleck screening library exercise trials. On test days, participants were asked to report to the testing laboratory in the morning following a 12-hour period without food. They were also asked to refrain from vigorous exercise in the 24-hour period prior to testing. On arrival to the laboratory, the participants

were provided with the respective supplement assigned for that session (GPLC or PL) and began a 90 minute resting period prior to testing. Supplementation Protocol The two high intensity exercise trials were Y-27632 cost performed under two conditions, one with GPLC and one without. The study supplements (GPLC, PL) were provided by Jarrow Formulas (Los Angeles, CA) in 750 mg capsules, with six capsules equivalent to the 4.5 gram daily dose. The GPLC was the USP grade nutritional product, GlycoCarn™ (Sigma Ta Health Sciences, S.p.A., Rome, Italy), which consists of a molecular bonded form of glycine and propionyl-L-carnitine.

The dosage of GPLC applied in this study is the same as that applied in previous research finding Ceramide glucosyltransferase elevated NOx levels at rest and in response to occlusive hyperaemia [13]. The PL capsules were visually identical and contained 750 mg of cellulose. The supplement assignments were blinded to both the research participants and the study investigators. Subjects ingested the respective 4.5 gram supplement with 8 ounces of water approximately 90 minutes prior to testing. Testing Protocol The assessment protocol consisted of five maximal effort 10-second cycle sprints performed with 1-minute active recovery periods between bouts. While Wingate type testing is typically performed using a single 30 second work period, repeated 10 second sprints have been used when testing exercise capacities similar to those required in relatively intense exercise. The sprints were performed using a Monarch 894E leg ergometer (Monarch, Varberb, Sweden) outfitted with pedal cages. The external resistance applied was equivalent to 7.5% of each subject’s body mass.