Moreover it has been suggested that CHO supplementation may increase neural drive thus leading to an attenuation of fatigue and increased exercise PFT�� cell line performance [36]. A limitation of the present was that the protocol simulated tennis match play conditions, however, it did not simulate tournament conditions in which athletes would play multiple matches with short recovery periods. Thus, the presented findings cannot be extrapolated to tournament conditions that include multiple matches. A second limitation

is that athletes received a high CHO diet (~60% daily energy expenditure; 8.33 g · kg-1 · day-1) during the experiment, which may have diminished the need for exogenous ingestion of CHO during the tennis match play. It is likely, that the high CHO diet and the rest period between matches (48 hours) was an ample protocol to fill glycogen stores, explaining the maintenance of blood glucose observed in the PLA condition. However, it is important to mention that previous investigations have reported that athletes do not achieve the daily CHO intake recommended during training and competitions [2, 42] and as a result Blasticidin S supplier liver and muscle glycogen stores might be compromised. In such scenario, CHO supplementation could be alternative

to provide energy and spare glycogen stores, delaying fatigue and attenuating performance decrement. Finally, the results of the present study should be interpreted with caution considering that the study’s sample consisted of well-trained athletes, who might have advanced physiological adaptations that could modulate the responses Methocarbamol observed (e.g. more efficient counter-regulatory hormonal response, greater hepatic glucose production, lower reliance on carbohydrates and higher utilization of lipids as energy substrate [43]), which may not otherwise occur in a less-advance athletic population. Conclusions The main finding of the present study were: first, CHO supplementation does not augment measures of tennis match play performance and, second, no significant difference in blood glucose was detected after CHO trial compared to a PLA during 180 minutes of simulated match

play, however there was a trend toward higher blood glucose in the CHO trial. It is possible that the metabolic demands of 180 minutes of tennis match play are not great enough to significantly lower blood glucose when players were fed a sufficient CHO diet (>8 g · kg-1·day-1). However, during prolonged matches or tournaments that require multiple matches in a 24-hour time span an athlete may benefit from CHO supplementation. Therefore, coaches and athletes should carefully assess the Selleckchem CX-6258 timing and requirements of a single match or a tournament and determine if CHO supplementation is necessary. Further research is necessary to investigate the effects of CHO supplementation during longer matches and in tournament-style play of multiple matches in a 24-hour time span to clarify recommendations. References 1.