Glucocorticoid mediated apoptosis is cell form specific To determine the apoptotic result that glucocorticoids have on numerous cell lines, we treated CEM C7 14, CEM C1 15, A549, and HeLa cell lines with dexametha sone and carried out flow cytometry, The lymphoblastic leukaemia CEM C7 buy inhibitor 14 cells are delicate to glucocorticoid induced apoptosis, whereas the CEM C1 15 cell line is often a chosen in vitro subclone with the CEM C7 14 resistant to GC mediated apoptosis, Though A549 lung carcinoma cells happen to be extensively studied with respect to GR, inconclusive final results are actually reported in terms of their response to glucocorticoid induced apoptosis, even though preceding reviews have proven that ectopic GR expression induces apoptosis in these cells, In CEM C1 15 and A549 the percentage of cells while in the sub G1 phase was typically below 10%, and a slight lessen in S phase on the cell cycle 48 h soon after dexamethasone treatment method was observed, Inside the situation of CEM C7 14 cells, prolonged hormone treatment method resulted in the boost with the apoptotic population and concomitant reduce within the variety of cells entering the S phase of the cell cycle, The impact of phosphorylation within the apoptotic profile of CEM C7 14 cells was investigated by treating UV irra diated or not irradiated cells with dexamethasone to the indicated occasions and subjecting them to FACS analy sis.
As shown in Figure 8A the longer treatment method RITA with dexamethasone improved the Sub G1 population from 21% in 0 h to 56% in 48 h dexamethasone handled cells.